Some Immunohistochemical Studies on the Epididymal Duct in the Donkey (Equus asinus)

Document Type : Original Article

Author

Department of Cytology and Histology, Faculty of Veterinary Medicine, Minufiya University, Sadat City Branch, Egypt.

Abstract

This work aimed to study the regional distribution pattern of some biologically active proteins of the epididymal duct of donkey through application of immune-histochemistry (IHC). Paraffin-embedded sections from different regions of the epididymal duct were used. Primary antibodies against S100, angiotensinconverting enzyme (ACE), galactosyl- transferase (GalTase), vascular endo-thelial growth factor (VEGF), α-smoothmuscle actin (α-SMA) and connexin 43(Cx43) were used. The immunehistochemical findings exhibited a regional-specific, distribution pattern; different from other domestic mammals. Apical cells in the epididymal epithelium ex- pressed a moderate to strong immune-staining with all of S100, ACE and Gal-Tase. Basal cells exhibited mode-rate to strong immunoreaction with both of GalTase and VEGF.

Keywords