Document Type : Original Article
Authors
1
Department of Histology and Cytology, Faculty of Veterinary Medicine, Benha University, Egypt.
2
Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Egypt.
3
Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Egypt & Department of Medical Laboratories and Medical Biotechnology, Faculty of Applied Medical sciences, Taif University, Turabuha branch, KSA.
4
Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, Egypt.
5
Department of Pathology, Faculty of Veterinary Medicine, Zagazig University,
6
Department of Genetics, Faculty of Agriculture, Ain Shams University, Egypt.
Abstract
The pituitary adenohypophysis was obtained from thirty buffaloes-cows, their age's ranges from one month to12 years. Sections of adenohypophysis tissues were immunostained for α, βa, and βb subunits of inhibin hormone. Positive immuno-staining specific for the α subunits of inhibin were detected in the cells of the follicles of the adenohypophysis in all ages. Moreover, immunostaining specific for the inhibin βa subu-
nits were strong positive at one month, weak positive at 4.5, 8 and 12 years and were negative at 8 months and 1.5 years. However, the immunostaining specific for the in- hibin βb subunits were positive at one and 8 months, 8 and 12 years and weak positive at 4.5 years and negative at 1.5 year. RT-PCR analysis revealed that both α and βb subunits are expressed in all ages except at 1.5 years old animals while βa subunit is only expressed at young age.
Keywords
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