Some Comparative Immunohistochemical Studies on the Ductus Deferens in the Donkey (Equus asinus) and Water Buffalo Bull (Bubalus bubalis).

Alkafafy, Mohamed; Attia, Hossam; Rashed, Reda; Kandiel, Mohamed

doi:10.21608/jva.2010.42681

Some Comparative Immunohistochemical Studies on the Ductus Deferens in the Donkey (Equus asinus) and Water Buffalo Bull (Bubalus bubalis).

Document Type : Original Article

Authors

¹ Department of Cytology and Histology, Faculty of Veterinary Medicine, Minuofiya University, Sadat City Branch, Egypt

² Department of Histology and Cytology, Faculty of Veterinary Medicine, Benha University, Tokheh, Kaliobia, Egypt.

³ Department of Anatomy and Embryology, Faculty of veterinary Medicine, Minoufiya University, Sadat City branch, Egypt.

⁴ Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Tokheh, Kaliobia, Egypt.

10.21608/jva.2010.42681

Abstract

The current work aimed to apply immu- nohistochemistry (IHC) to evaluate the differential distribution pattern of some biologically active proteins in the ductus deferens (DD) in two different animal species common in the Egyptian farms. Immunolocalization of S100, angiotensin converting enzyme (ACE), α- smooth muscle actin (α-SMA), connexin 43 (Cx43) and galactosyltransferase (GalTase) was practiced on paraffin- embedded sections of DD from clinically healthy adult five donkeys and five water buffalo bulls. The DD, in both species, was lined by a folded, high cuboidal (in donkey) or tall columnar (in buffalo) pseudostratified epithelium with stereocilia. IHC revealed that all antibodies used, displayed variable reactivities in the two species. Nerves and the periductal smooth muscles expressed a strong immunoreactivity (IR) for S100and α-SMA respectively; both in the donkey and in the buffalo. Stereocilia of principal cells showed a marked ACE- immunostaining in the donkey but not in the buffalo. Cx43-binding sites were found between the basal portions of the ductal epithelium and the subjacent interstitium; as well as among the periductal smooth muscle cells (SMCs) only in the buffalo bulls. Distinct GalTase- binding sites were found in the ductal epithelium only in the donkey. In conclusion the present findings exhibited a species-specific pattern of distribution for most of proteins under study, sug- gesting a variable functional signific- ance of the DD in the different animal species.

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