Histological, immunohistochemical and Scanning Electron Microscopic Studies on the Cerebellum of Dromedary Camel (Camelus dromedarius)

Document Type : Original Article

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Department of Histology and Cytology. Faculty of Veterinary Medicine, Zagazig University

Abstract

Little researches are known about the cerebellum of camel. This work aimed to study the morphology and histology of the cerebellum in ten camels by using histological paraffin sections and scanning electron microscope. Also, avidinbiotin complex peroxi- dase (ABC) method for the demonstration of glial fibrillary acidic protein (GFAP), and S-100 protein.
The results revealed that the cerebel- lum was formed from cortex and medulla. The cerebellar cortex consists of three layers, outer molecular layer, middle Purkinje cell layer and inner granular layer. The immunohisto- chemical findings show that S100 and GFAP immunoreactivity was found in the three layers of cerebellar cortex and in the medulla. The cells of the molecular layer, Purkinje cells and the granule cells were immunostained with anti-S-100 protein, in addition the glia cells and their processes, which were detected in form of network were also strongly immunostained. GFAP immunoreactivity in the molecular layer was detected but less dense than S100-immunereactivity. The Purkinje cells were surrounded by brown network of GFAP immuno- reactive glial cells. In the granular layer, the GFAP immunoreactivity showed similar reaction to those of S100 - immunoreactivity. Scanning electron microscopic findings rvealed that the dorsal surface of the cerebellum showed two elevated ridges (folia) in between groove (sulci). The dorsal surface of cerebellar cortex showed a corrugation, which resembles the irregular bleb like protrusions.Conclusions: the results obtained from this study revealed that the cerebellum of camel contains immunoreactive S100 protein and GFAP.

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